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Effective Oral RNA Interference (RNAi) Administration to Adult Anopheles gambiae Mosquitoes

Article DOI: 10.3791/63266-v • 07:48 min • March 1st, 2022
March 1st, 2022 •
1,2, 1, 3, 4,5, 5,6, 1, 5,7,8
1Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, 2Department of Entomology, Cornell University, 3Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, 4Department of Cell Biology, Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology, Johns Hopkins School of Medicine, 8Biomedical Sciences Department, Idaho College of Osteopathic Medicine

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The oral administration of dsRNA produced by bacteria, a delivery method for RNA interference (RNAi) that is routinely used in Caenorhabditis elegans, was successfully applied here to adult mosquitoes. Our method allows for robust reverse genetics studies and transmission-blocking vector studies without the use of injection.

Tags

Oral RNA Interference Gene Silencing Reverse Genetics Adult Mosquitoes Anopheles Gambiae Double Strand RNA Delivery Method Escherichia Coli Bacterial Culture Gene Function Study Anopheles Albinamus Dilution Double Stranded RNA Production IPDG Optical Density Centrifugation
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