JoVE Journal
Developmental Biology
Developmental Biology
需要订阅 JoVE 才能查看此 内容.
章节
总结概括
The present protocol describes an enhanced method to increase the co-expression of PDX1 and NKX6.1 transcription factors in pancreatic progenitors derived from human pluripotent stem cells (hPSCs) in planar monolayers. This is achieved by replenishing the fresh matrix, manipulating cell density, and dissociating the endodermal cells.