快速将重建的细胞骨架封装到巨大单层囊泡内
4.4K 次观看
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被引用 20 次
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07:48 分钟
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2021年11月10日
DOI :10.3791/63332-v
2021年11月10日
•4.4K 次观看
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally
本文介绍了一种快速制备包封细胞骨架蛋白的巨型单层囊泡的简单方法。该方法适用于在受限环境中从头重建细胞骨架结构以及研究细胞骨架与膜之间的相互作用。
Chapters in this video
0:05
Introduction
0:48
Preparation of Oil-Lipid-Mixture
2:12
Vesicle Generation
5:42
Imaging and 3D image Reconstruction
6:53
Results: Encapsulation of Actin Bundle Structures
7:08
Conclusion
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