一种基于 cryoSPARC、RELION 和 Scipion 的稳健单颗粒冷冻电子显微镜（cryo-EM）处理流程
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被引用 17 次
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13:43 分钟
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2022年1月31日
2022年1月31日
•本文介绍了如何有效利用三种冷冻电镜处理平台——cryoSPARC v3、RELION-3 和 Scipion 3，构建一个适用于多种单颗粒数据集的单一且稳健的工作流程，以实现高分辨率结构解析。
Chapters in this video
0:04
Introduction
0:56
Creating a New CryoSPARC v3 Project and Importing Data
1:34
CryoSPARC v3: Movie Alignment and Contrast Transfer Function (CTF) Estimation
2:52
CryoSPARC v3: Manual and Template-Based Particle Picking
4:21
CryoSPARC v3: 2D Classification
4:52
CryoSPARC v3: Ab-initio Reconstruction and Homogeneous Refinement
5:31
Exporting Particle Coordinates from CryoSPARC v3 and Importing Them to RELION-3 Using PyEM
5:56
RELION-3: Particle Extraction and 2D Classification, 3D Refinement, Mask Creation, and Post-Processing
7:41
RELION-3: Polishing Training, Particle Polishing, CTF, and Per-Particle Refinements
9:15
Transferring RELION-3 Particle Coordinates and 3D Map to Scipion 3
10:05
Scipion 3: High-Resolution Refinement
11:21
Results: Using cryoSPARC v3, RELION-3, and Scipion 3 to Obtain a High-Resolution 3D Reconstruction of the Adeno-Associated Virus
12:52
Conclusion
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