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Immunology and Infection

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Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Article DOI: 10.3791/63395-v • 11:11 min • February 11th, 2022
February 11th, 2022 •
*1, *1,2, 1, 1, 3, 4, *1,5,6, *1,2
1Center for Innovative Medical Devices and Sensors (REDDI Lab), Clemson University, 2Department of Bioengineering, Clemson University, 3Swann Medicine, 4Student Health Services, Clemson University, 5Department of Chemical & Biomolecular Engineering, Clemson University, 6Department of Chemical & Biomolecular Engineering, University of Delaware
* These authors contributed equally

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The protocol describes a SARS-CoV-2 diagnostic method that utilizes open-source automation to perform RT-qPCR molecular testing of saliva samples. This scalable approach can be applied to clinical public health surveillance as well as to increase the capacity of smaller university laboratories.

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SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy Open-Source Pipetting Robots Low Cost COVID-19 Diagnostics Test Workflow Community Screening Applications Liquid Handling Robots Thermocyclers Software Saliva Testing Diagnostic Results Automated Parallel Workflows Scalability Equipment Requirements Personnel Requirements Decontamination Sample Arrival Sample Barcode Heat Treatment Sample Loading Spreadsheets 384 Well Plate
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