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Storskalig, automatiserad produktion av fett-härledda stamcellsfäroider för 3D-bioprintning

Article DOI: 10.3791/63430-v • 07:40 min • March 31st, 2022
March 31st, 2022 •
1,2,3, 1,2,4, 1,2,4, 1,2, 2,3,4,5, 1,2,3,4
1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio), Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans), Unigranrio, 4Post-graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School, Fluminense Federal Fluminense

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Här beskriver vi storskalig produktion av fett-härledda stroma / stamcell (ASC) sfäroider med hjälp av ett automatiserat pipetteringssystem för att så cellsuspensionen, vilket säkerställer homogenitet av sfäroid storlek och form. Dessa ASC-sfäroider kan användas som byggstenar för 3D-bioprintningsmetoder.

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