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Chapters
- 00:04Introduction
- 00:54An in Vitro Inoculation System Based on Petri Dishes
- 02:55An in Vitro Inoculation System Based on Plastic Cups
- 04:23A Soil Based Inoculation System in Pots
- 06:07Results: Infection of Arabidopsis Roots
- 07:29Conclusion
This protocol presents a detailed summary of strategies to inoculate plant roots with soil-borne microbes. Exemplified for the fungi Verticillium longisporum and Verticillium dahliae, three different root infection systems are described. Potential applications and possible downstream analyses are highlighted, and advantages or disadvantages are discussed for each system.
