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DOI: 10.3791/63446-v
Please note that some of the translations on this page are AI generated. Click here for the English version.
该协议详细介绍了用土壤微生物接种植物根部的策略。以真菌黄霉菌和大丽黄萎病为例,描述了三种不同的根部感染系统。重点介绍潜在应用和可能的下游分析,并讨论每个系统的优缺点。
虽然无数微生物在植物根部定殖,但这些相互作用很难分析,因为它们发生在地下。为了促进这一点,该协议收集提供了用土壤传播的微生物接种植物根部的策略的概述。根接种是研究根部微生物相互作用的基础,并且通过使用以下技术,可以在分子,细胞学或组织学水平上研究相互作用。
我们解释了三种使用疣作为根入侵微生物的接种系统,以及模型植物拟南芥。然而,将这些方法转移到其他植物如番茄或油菜,以及其他根部微生物,例如有益的偶然性,是可能的。通过在PDB中培养菌丝体并诱导孢子化和Czapek葡萄糖汤,提前准备黄萎病接种物。
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