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Neuroscience

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JoVE Journal Neuroscience
Primære kulturer af rotteastrocytter og microglia og deres anvendelse i undersøgelsen af amyotrofisk lateral sklerose
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Primære kulturer af rotteastrocytter og microglia og deres anvendelse i undersøgelsen af amyotrofisk lateral sklerose

Article DOI: 10.3791/63483-v • 09:36 min • June 23rd, 2022
June 23rd, 2022 •
1, 1, 1, 1
1Center for Laser Microscopy, Institute for Physiology and Biochemistry “Jean Giaja”, University of Belgrade Faculty of Biology

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Vi præsenterer her en protokol om, hvordan man forbereder primære kulturer af gliaceller, astrocytter og microglia fra rottekortika til time-lapse videobilleddannelse af intracellulær Ca2+ til forskning i patofysiologi af amyotrofisk lateral sklerose i hSOD1G93A rottemodellen.

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Neurovidenskab udgave 184
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