JoVE Neuroscience is a multidisciplinary section devoted to investigations of the structure, function, physiology, and pathophysiology of the brain and nervous system. Included methodologies range from molecular and cellular level studies to full central and peripheral neural systems. Potential treatment platforms and surgical techniques for neurological diseases and disorders are also presented in this section.

Neuroscience

JoVE Medicine serves as a connection between basic research and clinical applications. Articles may present medical procedures, case studies or clinical trial methodologies. Included studies are often modeled towards predictive, preventative, personalized, and practical approaches to treatment of disease. The goal of research methodologies in this section is to improve overall patient care and prognosis.

Medicine

JoVE Immunology and Infection includes content from a variety of fields working toward a shared initiative of improving global health. Techniques evaluating the biological response to pathogens from the molecular to organismal level are presented as well as therapeutic agents and their efficacy in treating disease.

Immunology and Infection

JoVE Genetics publishes video articles demonstrating experimental approaches to investigate gene function and regulation. This includes methods to study gene expression, mutagenesis, epigenetics, genetic determinants of development, genetic disorders, population genetics and evolution.

Genetics

JoVE Environment is dedicated to research methodologies that address environmental concerns and seek to better understand Earth's ecosystem.  Special consideration is given to experimental methodologies that assess society’s impact on the environment, suggest solutions for protecting Earth’s resources, and develop sustainable fuel sources. Moving towards an environmentally conscious planet is a collaborative effort with contributions from both life and physical scientists.

Environment

JoVE Engineering encompasses a broad range of experimental and instrumental techniques utilized in physics research. Investigations in this area strive to address and answer a broad range of scientific questions, such as device mechanisms and efficiencies, using physical tools. This approach often requires a combination of specialties, and research in this area tends to be interdisciplinary with contributions from mechanical, electrical, and chemical engineers.

Engineering

JoVE Developmental Biology publishes research methodologies in the field of developmental biology. This includes methods that are used to study biological development in vitro and in vivo at the molecular, cellular, tissue, organ and whole organism levels.

Developmental Biology

JoVE Chemistry surveys a broad scope of chemical disciplines. This collection includes fundamental and applied research that investigates molecular interactions, properties, and structure spanning in the following core divisions: analytical, organic, organometallic, inorganic, bioinorganic, and biochemistry. This section also includes the design and preparation of materials and small molecules for advanced or unique applications.

Chemistry

JoVE Cancer Research illustrates experimental approaches in biomedical research and clinical practice aimed at understanding, detecting, treating and preventing cancer. The video articles in this section demonstrate methods to study the cellular and molecular mechanisms of cancer, animal models of cancer research, development of new therapies and diagnostics, tumor imaging, chemical and radiation treatments, surgical procedures and improvements in therapy. These video articles offer researchers and clinical practitioners an efficient way to learn the technologies and experimental methods in cancer research.

Cancer Research

JoVE Biology welcomes all general biology research methodologies. Content in this section canvases all fields of cell, molecular, and organismal biology, ranging from new applications of standard techniques to novel approaches aimed at understanding the functions of life and living organisms. This diverse section includes, but is not limited to, techniques in physical biology, cellular biochemistry, genetics, physiology, systems biology and a combination of eukaryotic and prokaryotic model systems.

Biology

JoVE Bioengineering merges both physical and life sciences to understand and predict biological processes. Applying physical science tools to life science questions allow for the discovery of better technologies to measure, diagnose, and clinically treat disease.

Bioengineering

JoVE Biochemistry publishes experiments that investigate the structure, function, and interactions of biological molecules. The video articles in this section demonstrate methods to study nucleic acids, proteins, carbohydrates and lipids as well as enzyme function, protein folding, molecular interactions and biochemical reactions.

Biochemistry

JoVE Behavior documents observational and experimental techniques that seek to understand human and animal behavior. Work in this field includes analysis of genetic causes and physiological responses to internal or external stimuli as well as the implicated brain regions.

Behavior

This protocol describes a two-phase surgical method to create a large, resealable, dura-sparing cranial window in mice. This technique enables chronic, multi-modal electrophysiological recordings from distributed, deep-brain networks, such as the Default Mode Network, over several weeks.

Surgical Protocol for a Large, Resealable Cranial Window Enabling Longitudinal, Multi-Modal Electrophysiology Recordings of Mouse Default Mode Network

We present a protocol to selectively permeabilize plasma membrane, enabling targeted delivery of substrates and inhibitors to assess mitochondrial oxidative phosphorylation (OXPHOS) in cultured cells, overcoming plasma membrane transport barriers that typically hinder such analysis. Using this technique, we demonstrated how OXPHOS function is modulated by muscarinic agonists and antagonists.

Cholinergic Ligand&#8211;dependent Modulation of Oxidative Phosphorylation Coupling in Digitonin-permeabilized BE(2)-C Neuroblastoma Cells

This protocol describes the workflow of robotically delivered fMRI-guided personalized transcranial magnetic stimulation therapy for treatment-resistant depression. Clinical implementation using this protocol is feasible, and open-label results support the real-world effectiveness of this approach.

Robotically Delivered fMRI-Guided Personalized Transcranial Magnetic Stimulation Therapy for Treatment-Resistant Depression

This protocol presents a step-by-step instruction to create a neonatal brachial plexus injury in rats to be used as a model in brachial plexus birth injury research.

Development of a Neonatal Rat Model for Brachial Plexus Birth Injury

This study employs ERPs to investigate how agent type (human/AI) and language style (humorous/rational) influence ride-hailing service recovery satisfaction, aiming to uncover neurocognitive mechanisms.

Humor or Rationality? The Neural Mechanisms of How Agent Type and Language Style Influence Satisfaction with Ride-Hailing Service Failure Recovery

This study presents a standardized and reproducible protocol for implementing the Spatial-Temporal-Frequency EEG analysis tool (STFEEG) for motor imagery EEG decoding, incorporating configurable spatial-temporal-frequency segmentation, Common Spatial Patterns (CSP)-based feature extraction, multiple classification algorithms, and visualization capabilities.

STFEEG-Tool: A Spatial-Temporal-Frequency EEG Analysis Tool for Motor Imagery Brain-Computer Interfaces

Here, we describe a standardized protocol for motor mapping using nTMS combined with diffusion tensor imaging (DTI)-based reconstruction of the corticospinal tract (CST). The protocol is reproducible, clinically feasible, and readily integrable into routine clinical workflows, providing a robust and valuable framework for motor pathway assessment, neuroplasticity research, and rehabilitation planning.

A Standardized Protocol for Functional Motor Mapping Using Navigated Transcranial Magnetic Stimulation

This article details intrathecal delivery methods for CNS-targeted therapeutics in adult mice, focusing on protocol precision and enabling repeated injection. It includes findings from a validation study where antisense oligonucleotides reduced mutant SOD1 expression in SOD1-ALS model mice, supporting the utility of the approach in preclinical research on neurodegenerative diseases.

Lumbar Intrathecal Injection of SOD1-ASOs for Precise CNS Targeting and Predictive Efficacy in Human SOD1-G93A ALS Mice

An innovative method to build expandable brain matrices to cut either coronal or sagittal slices is described for application to neonatal piglets. This budget-friendly approach utilizes acrylic plates carved using templates from agarose gel brain molding, applies to multiple species, and allows for expansion to accommodate brain growth.

Fabrication of an Expandable Brain Matrix Customizable Across Developmental Stages

We introduced a novel imaging technique called Optical Multilayer Interference Tomography (OMLIT), which enables unbiased imaging of all cells in brain specimens at the mesoscale and can be seamlessly integrated into the imaging workflow of tape-based serial scanning electron microscopy on the same sample.

Journal - Neuroscience