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A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

Article DOI: 10.3791/63504-v • 06:45 min • February 7th, 2022
February 7th, 2022 •
*1, *1, 1,2, 3, 1
1Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, 2School of Medicine, Chongqing University, 3Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University
* These authors contributed equally

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In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique.

Tags

Modified Cuff Technique Mouse Cervical Heterotopic Heart Transplantation Model Cardiac Allografts Immunologic Parasagittal Rejection Mechanisms Long-term Survival Cervical Transplantation Surgery Vessel Anastomosis Blood Supply Venous Reflux Recipient Mouse Heating Pad Surgical Area Incision Right Mandibular Angle Tail End External Jugular Vein Electro-coagulator Micro-forceps Atruamatic Microvascular Clamp Ligate Polyurethane Barbed Cuff Bevel End Superficial Grooves Ligation Suture
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