/

/

量化Cu（II）和肽残基在存在和不存在发色团的情况下的结合相互作用

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

JoVE 杂志
生物化学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志生物化学

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

量化Cu（II）和肽残基在存在和不存在发色团的情况下的结合相互作用

Please note that all translations are automatically generated. Click here for the English version.

2,411 Views

•

11:38 min

•

April 05, 2022

DOI:

10.3791/63668-v

DOI:

10.3791/63668-v

•

11:38 min

April 05, 2022

•

2381 Views

Sohee Choi¹, Jessica A. San Juan², Marie C. Heffern², Michael J. Stevenson¹

¹Department of Chemistry,University of San Francisco ²Department of Chemistry,University of California, Davis

Summary

本文重点介绍使用电子吸收光谱和等温滴定量热法来探测和量化Cu（II）与肽和蛋白质结合的热力学。

Explore More Videos

Cu(II)Peptide Residues Binding Interactions Chromophores Electronic Absorption Spectrophotometer Orthogonal Techniques Metal Peptide Interactions Absorption Spectrum Substoichiometric Amount Ultrapure Water Double Beam Spectrophotometer Sample Cell Holder Mixing Solution

Related Videos

Read Article