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Establecimiento de organoides pulmonares humanos y diferenciación proximal para generar organoides maduros de las vías respiratorias
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Establecimiento de organoides pulmonares humanos y diferenciación proximal para generar organoides maduros de las vías respiratorias

Article DOI: 10.3791/63684-v • 10:12 min • March 23rd, 2022
March 23rd, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2,3
1Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, 2Centre for Virology, Vaccinology and Therapeutics, Hong Kong Science and Technology Park, 3State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong

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El protocolo presenta un método para derivar organoides pulmonares humanos a partir de tejidos pulmonares primarios, expandir los organoides pulmonares e inducir la diferenciación proximal para generar organoides de las vías respiratorias 3D y 2D que fenopicon fielmente el epitelio de las vías respiratorias humanas.

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Biología Número 181
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