Establishment of Zebrafish Patient-Derived Xenografts from Pancreatic Cancer for Chemosensitivity Testing

Alice Usai; Gregorio Di Franco; Chiara Gabellini; Luca Morelli; Vittoria Raffa

doi:10.3791/63744

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Establishment of Zebrafish Patient-Derived Xenografts from Pancreatic Cancer for Chemosensitivity Testing

建立胰腺癌斑马鱼患者来源的异种移植物进行化学敏感性测试

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06:59 min

May 12, 2023

DOI: 10.3791/63744-v

Alice Usai¹, Gregorio Di Franco², Chiara Gabellini¹, Luca Morelli², Vittoria Raffa¹

¹Department of Biology,University of Pisa, ²General Surgery Unit, Department of Translational Research and New Technologies in Medicine and Surgery,University of Pisa

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临床前模型旨在提高癌症生物学知识并预测治疗效果。本文描述了基于斑马鱼的患者来源的带有肿瘤组织碎片的异种移植物（zPDX）的产生。zPDXs用化疗治疗，根据移植组织的细胞凋亡评估其治疗效果。

斑马鱼患者来源的异种移植物是一种强大的临床前模型，用于预测癌症患者体内的化学敏感性特征。该协议描述了从患者手术标本开始的模型生成。通过异种移植小组织碎片而不是将细胞分离到斑马鱼胚胎中，可以维持肿瘤微环境，这是肿瘤进展和化疗反应的关键步骤。

该模型的最大优势在于它有可能在短短一周的临床相关时间范围内预测患者的预后，作为个性化医疗的工具。通过用五毫升新鲜肿瘤培养基洗涤从患者收集的整个肿瘤组织开始样品处理。使用塑料巴斯德移液器上下移液10次。

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癌症研究第195期斑马鱼头像临床前模型联合临床试验肿瘤移植化学敏感性全安装免疫荧光