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JoVE Journal Cancer Research
Mantenimiento de la diversidad celular del glioblastoma humano ex vivo mediante cultivo de organoides tridimensionales
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Mantenimiento de la diversidad celular del glioblastoma humano ex vivo mediante cultivo de organoides tridimensionales

Article DOI: 10.3791/63745-v • 07:11 min • August 25th, 2022
August 25th, 2022 •
1, 2, 1, 2
1Department of Neurological Surgery, Cleveland Clinic, 2Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic

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Aquí, describimos un método para generar organoides de glioblastoma (GBM) a partir de muestras primarias de pacientes o cultivos celulares derivados de pacientes y mantenerlos hasta la madurez. Estos organoides GBM contienen poblaciones celulares fenotípicamente diversas y recrean microambientes tumorales ex vivo.

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Investigación del cáncer Número 186
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