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Visualizing Scar Development Using SCAD Assay – An Ex-situ Skin Scarring Assay
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生物学
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JoVE 杂志 生物学
Visualizing Scar Development Using SCAD Assay – An Ex-situ Skin Scarring Assay

Visualizing Scar Development Using SCAD Assay – An Ex-situ Skin Scarring Assay

DOI:
10.3791/63808-v

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07:40 min

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April 28, 2022

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, , , ,
1Institute of Regenerative Biology and Medicine,Helmholtz Zentrum München, 2School of Medicine, Klinikum Rechts der Isar, Department of Plastic and Hand Surgery,Technical University of Munich

Chapters

  • 00:05Introduction
  • 01:03Scar like Tissue in A Dish (SCAD) Preparation
  • 02:50SCAD: Tissue Culture & Live Imaging of SCADs
  • 04:23Tissue Harvesting and 2D/3D Immunofluorescence Staining
  • 05:07Results: An Ex Situ Skin Scarring Assay to Visualize Scar Development
  • 06:43Conclusion

Summary

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This protocol describes the generation of a skin-fascia explant termed "SCar like tissue in A Dish" or SCAD. This model allows unprecedented visualization of single fibroblasts during scar formation.

Tags

SCAD AssayEx Situ Skin Scarring AssayFibroblast MigrationScar FormationWound RepairHigh-throughput ScreeningSkin ExplantsPost-natal Day Zero Or Day One SkinFull-thickness Dorsal Back SkinPanniculus Carnosus MuscleDMEM F-12 MediumHanks Balanced Salt Solution96-well PlateLive ImagingLow-melting Agarose Solution

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