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JoVE Journal Developmental Biology
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Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Article DOI: 10.3791/63834-v • 07:26 min • April 1st, 2022
April 1st, 2022 •
*1, *1, 1
1Department of Craniofacial Biology, School of Dental Medicine, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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The protocol presents a method for isolating whole cell protein lysates from dissected mouse embryo facial processes or cultured mouse embryonic palatal mesenchyme cells and performing subsequent western blotting to assess phosphorylated protein levels.

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Isolation Whole Cell Protein Lysates Mouse Facial Processes Cultured Palatal Mesenchyme Cells Phosphoprotein Analysis Dynamics Of Phosphoproteins Craniofacial Development Dephosphorylation Protein Isolation Physiologically Relevant Context Mouse Embryonic Palatal Mesenchyme Cells Phosphatase Inhibitors Lysis Buffers Phosphorylated Proteins Mouse Body Dissecting Board Ventral Side 70% Ethanol Abdominal Cavity Vaginal Opening Semken Forceps Straight Blade Surgical Scissors Uterine Horns Oviducts Cervix Histology PBS
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