自下而上法 体外 检测Septin超微结构组织、膜重塑及曲率敏感行为的方法
2.2K 次观看
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被引用 2 次
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09:09 分钟
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2022年8月17日
DOI :10.3791/63889-v
2022年8月17日
•2.2K 次观看
Brieuc Chauvin*1 , Koyomi Nakazawa*1 , Alexandre Beber1,7 , Aurélie Di Cicco1 , Bassam Hajj1 , François Iv2 , Manos Mavrakis2 , Gijsje H. Koenderink3 , João T. Cabral4 , Michaël Trichet5 , Stéphanie Mangenot*6 , Aurélie Bertin*1
1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, 2Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 4Department of Chemical Engineering, Imperial College London, 5Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Service de microscopie électronique (IBPS-SME), 6Laboratoire Matière et Systèmes Complexes (MSC), Université Paris Cité, 7Institute of Biotechnology, Czech Academy of Sciences, BIOCEV
* These authors contributed equally
Septins 是细胞骨架蛋白。它们可与脂质膜相互作用，并能够在微米尺度上感知甚至产生膜曲率。本实验方案中，我们描述了自下而上的体外（in vitro）方法，用于分析膜形变、对曲率敏感的 septin 结合行为以及 septin 丝状结构的超微结构。
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Chapters in this video
0:05
Introduction
0:49
Designing Wavy NOA (Norland Optical Adhesive) Replica from Wavy PDMS (Polydimethylsiloxane) Patterns
1:49
Generation of a Supported Lipid Bilayer and Protein Incubation
3:22
Sample Fixation, Sample Dehydration, Critical Point Drying
4:43
Sample Mounting and Coating
5:54
Acquire the Images Using a Field-Emission SEM (FE-SEM) Microscope
6:56
Results: Analysis of the Self-Assembly of Septins at the Lipid Membrane
8:27
Conclusion
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