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JoVE Journal Neuroscience
En vevsryddingsmetode for nevronal avbildning fra mesoskopisk til mikroskopisk skala
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En vevsryddingsmetode for nevronal avbildning fra mesoskopisk til mikroskopisk skala

Article DOI: 10.3791/63941-v • 07:20 min • May 10th, 2022
May 10th, 2022 •
1,2, 1,2,3, 1,2,4,5, 2,3, 6, 1,2,7
1Department of Neuroanatomy, Juntendo University Graduate School of Medicine, 2Department of Cell Biology and Neuroscience, Juntendo University Graduate School of Medicine, 3Advanced Research Institute for Health Sciences, Juntendo University, 4Department of Neuroscience, Graduate School of Medicine, Kyoto University, 5Japan Society for the Promotion of Science, 6Department of Oral Anatomy and Neurobiology, Graduate School of Dentistry, Osaka University, 7Department of Multi-Scale Brain Structure Imaging, Juntendo University Graduate School of Medicine

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Protokollen gir en detaljert metode for nevronal avbildning i hjerneskive ved hjelp av en vevsryddingsmetode, ScaleSF. Protokollen inkluderer hjernevevsforberedelse, vevsavklaring, håndtering av ryddede skiver og konfikal laserskanning av mikroskopisk avbildning av nevronstrukturer fra mesoskopisk til mikroskopisk nivå.

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