用于基因治疗应用的造血干细胞和祖细胞CRISPR/Cas9基因编辑
3.8K 次观看
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被引用 6 次
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08:32 分钟
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2022年8月9日
DOI :10.3791/64064-v
2022年8月9日
•3.8K 次观看
Vigneshwaran Venkatesan*1,2 , Abisha Crystal Christopher*1,3 , Karthik V. Karuppusamy1,2 , Prathibha Babu1,2 , Manoj Kumar K. Alagiri1,2 , Saravanabhavan Thangavel1
1Centre for Stem Cell Research (CSCR), A unit of InStem Bengaluru, Christian Medical College campus, 2Manipal Academy of Higher Education, 3Thiruvalluvar University
* These authors contributed equally
本方案描述了一种优化的造血干细胞和祖细胞（HSPC）培养程序，用于高效移植基因编辑细胞 体内.
Chapters in this video
0:04
Introduction
0:53
Gene Editing of HSPCs
2:55
Transplantation of Gene-Edited HSPCs
4:39
Assessment of Short-Term Engraftment Potential
5:17
Assessment of Long-Term Engraftment Potential
7:01
Results: Evaluating the Efficiency of CRISPR/Cas9 Gene Editing of HSPCs
7:58
Conclusion
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