Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Michael  S. Xu; Golnaz Karoubi; Thomas K. Waddell; Siba Haykal

doi:10.3791/64068

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

在定制灌注生物反应器中获取和灌注-脱细胞猪血管化皮瓣

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10:56 min

August 1, 2022

DOI: 10.3791/64068-v

Michael S. Xu¹, Golnaz Karoubi^1,2, Thomas K. Waddell^1,3, Siba Haykal^1,4

¹Latner Thoracic Surgery Research Laboratories,Toronto General Hospital Research Institute, University Health Network, ²Institute of Laboratory Medicine and Pathobiology,University of Toronto, ³Division of Thoracic Surgery, Department of Surgery,University of Toronto, ⁴Division of Plastic and Reconstructive Surgery, Department of Surgery,University of Toronto

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该协议描述了通过在定制的灌注生物反应器中通过皮瓣脉管系统灌注十二烷基硫酸钠洗涤剂来手术获取和随后的血管化猪皮瓣的脱细胞。

该方法描述了三个血管化猪瓣的手术获取及其随后的灌注脱细胞。该方法可以帮助使用组织去细胞化和再细胞化方法再生猪瓣。该技术的主要优点是其多功能性，可以在易于组装的生物反应器设置中实现各种血管化猪瓣的大量脱细胞。

这里描述的技术可以广泛应用于其他血管化皮瓣，作为在重建手术中使用猪皮瓣的潜在组织工程解决方案。首先，通过测量大约 50 厘米的 LS16 铂涂层硅胶管来制作流入和流出管。将一端连接到黄色 3 停止泵管，另一端连接到无菌 2 毫升血清移液管，以将灌注液从洗涤剂储液槽输送到生物反应器室中。

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