利用MRI、组织透明化和光片显微镜对完整新生小鼠脑进行全脑单细胞成像与分析
3.7K 次观看
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被引用 2 次
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08:49 分钟
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2022年8月1日
DOI :10.3791/64096-v
2022年8月1日
•3.7K 次观看
Felix A. Kyere*1,2 , Ian Curtin*1,2 , Oleh Krupa1,2 , Carolyn M. McCormick1,2 , Mustafa Dere3 , Sarah Khan3,7 , Minjeong Kim7 , Tzu-Wen Winnie Wang4,5,6 , Qiuhong He4,5,6 , Guorong Wu3 , Yen-Yu Ian Shih4,5,6 , Jason L. Stein1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally
本方案描述了使用iDISCO+对完整小鼠脑组织进行磁共振成像、透明化处理和免疫标记的方法，随后详细介绍了利用光片显微镜进行成像，以及使用NuMorph进行后续分析的步骤。
Chapters in this video
0:05
Introduction
0:48
Light-Sheet Imaging
2:25
Image Processing Using NuMorph
4:12
Image Analysis
6:26
Results: Quantification of Nuclear Markers Within Mouse Brain After Imaging on a Light-Sheet Microscope Using NuMorph
7:57
Conclusion
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