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用于动态界面共聚焦显微镜可视化的微量天体测量仪
用于动态界面共聚焦显微镜可视化的微量天体测量仪
JoVE Journal
Engineering
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JoVE Journal Engineering
Microtensiometer for Confocal Microscopy Visualization of Dynamic Interfaces

用于动态界面共聚焦显微镜可视化的微量天体测量仪

Full Text
2,897 Views
08:05 min
September 9, 2022

DOI: 10.3791/64110-v

Steven V. Iasella1, Sourav Barman1, Clara Ciutara1, Boxun Huang1, Michael L. Davidson2, Joseph A. Zasadzinski1

1Department of Chemical Engineering and Materials Science,University of Minnesota, 2Department of Chemical Engineering,Carnegie Mellon University

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Please note that some of the translations on this page are AI generated. Click here for the English version.

本手稿描述了微量血压计/共聚焦显微镜的设计和操作,以便在可视化界面形态的同时测量界面张力和表面膨胀流变学。这提供了在技术和生理学中重要的界面的结构 - 属性关系的实时构建。

该方法将共聚焦显微镜与毛细管压力微张力仪相结合,创造了一种强大的工具,可用于以高空间和时间分辨率研究曲线流体界面。该技术可以通过同时测量高度弯曲界面上的表面特性和表面形态的共聚焦图像来检查表面活性材料的结构和功能关系。我们假设炎症产物抑制肺表面活性剂,导致与准确的呼吸窘迫综合征相关的呼吸问题。

该门可以研究肺表面活性剂的性质和形态以及经受此类材料的肺稳定性。首先,通过将毛细管的大侧放入细胞的顶部直到它推到细胞的下侧来组装 CPM 细胞。轻轻拧紧峰值连接器以固定毛细管,然后将微流体泵的管子连接到毛细管的大侧。

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工程 第187期 毛细管压力微量强度计 界面流变学 肺表面活性剂 共聚焦显微镜 表面形态学 微流体

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