构建人主动脉平滑肌细胞类器官芯片模型以模拟主动脉壁中的生物力学应变
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被引用 1 次
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11:47 分钟
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2022年7月6日
DOI :10.3791/64122-v
2022年7月6日
•3.2K 次观看
Mieradilijiang Abudupataer*1 , Xiujie Yin*1 , Bitao Xiang*1 , Nan Chen1 , Shiqiang Yan2 , Shichao Zhu1 , Yang Ming1 , Gang Liu1 , Xiaonan Zhou1 , Hao Lai1 , Chunsheng Wang1 , Kai Zhu1 , Jun Li1
1Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, 2Institutes of Biomedical Sciences and the Shanghai Key Laboratory of Medical Epigenetics, Shanghai Medical College, Fudan University
* These authors contributed equally
在此，我们开发了一种人主动脉平滑肌细胞类器官芯片模型，用于模拟 体内 人主动脉壁平滑肌细胞的生物力学应变
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Chapters in this video
0:04
Introduction
1:03
Primary Human Aortic Smooth Muscle Cell Isolation
2:53
Preparation of PDMS Chip
5:29
PDMS Chip Surface Treatment and Sterilization
6:12
Cell Seeding on the PDMS Chip and Cell Stretching Process
8:23
Preparation of the Mechanical Control System
9:30
Results: An HASMC‐Organ‐On‐A‐Chip Model to Precisely Control the Parameters of the Biomechanical Strain on the Aortic Wall
11:03
Conclusion
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