Optimization of Transesophageal Atrial Pacing to Assess Atrial Fibrillation Susceptibility in Mice

Matthew B. Murphy; Kyungsoo Kim; Prince J. Kannankeril; Katherine T. Murray

doi:10.3791/64168

JoVE Journal
Biology

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Optimization of Transesophageal Atrial Pacing to Assess Atrial Fibrillation Susceptibility in Mice

优化经食管心房起搏以评估小鼠心房颤动易感性

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08:05 min

June 29, 2022

DOI: 10.3791/64168-v

Matthew B. Murphy¹, Kyungsoo Kim¹, Prince J. Kannankeril¹, Katherine T. Murray¹

¹Departments of Medicine, Pediatrics and Pharmacology,Vanderbilt University School of Medicine

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Overview

This protocol describes the optimization of transesophageal atrial pacing to evaluate atrial fibrillation susceptibility in mice. The method allows for repeated testing in the same animal, enhancing the reproducibility of results.

Key Study Components

Research Area

Atrial fibrillation
Electrophysiology
Mouse models

Background

Understanding atrial fibrillation's electrophysiologic properties.
The importance of protocol standardization for experimental reproducibility.
Effects of pacing-induced parasympathetic stimulation on arrhythmias.

Methods Used

Transesophageal atrial pacing
Mice
ECG recording and analysis

Main Results

Optimal settings for pacing were established.
Data showed variations in AF susceptibility across different testing conditions.
Identified factors that could confound results, such as parasympathetic stimulation.

Conclusions

The study presents a refined protocol for assessing atrial fibrillation in mice.
It underlines the significance of meticulous protocol design in electrophysiological research.

Frequently Asked Questions

What is transesophageal atrial pacing?

It is a technique used to induce and study atrial fibrillation in experimental models, particularly in mice.

Why is it essential to optimize experimental parameters?

Optimizing parameters enhances the accuracy and reproducibility of research findings related to atrial fibrillation.

How can parasympathetic stimulation affect results?

It can lead to inadvertent induction of arrhythmias, confounding the interpretation of data regarding atrial fibrillation susceptibility.

What age should mice be for optimal testing?

Mice should be at least 12 weeks old for acquired models and start biweekly pacing at 8 weeks for genetic models.

What measures are taken to analyze atrial fibrillation?

Electrophysiologic parameters and duration of AF episodes are recorded, allowing for comprehensive analysis of susceptibility.

Why is serial testing important?

It allows researchers to assess changes in AF susceptibility over time in the same animal, improving data integrity.

Is this method applicable to genetic studies?

Yes, it is specifically designed to assess both genetic and acquired models of atrial fibrillation.

本协议描述了使用经食管心房起搏评估小鼠心房颤动易感性时实验参数的优化。

使用经食管心房起搏的程序性电刺激可以确定小鼠的电生理学特性和对心房颤动的易感性。在这里，我们描述了一种标准化的实验方案开发程序，可以优化重现性。经食管心房起搏方法通常是一种生存过程，因此可以在同一动物中进行连续测试。

方案开发的重要组成部分包括初始托盘研究，以优化所研究模型的实验参数，以及识别由起搏诱导的副交感神经刺激引起的心律失常。对于遗传模型，从八周龄开始对小鼠进行每两周一次的心房起搏，以确定AF易感性的最佳时期，研究两性，因为一个人可能不会发展出AF表型。对于获得的模型，在小鼠达到身体成熟后进行起搏。

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