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Chapters
- 00:04Introduction
- 01:05Preparation of Retinal Explants
- 03:08Primate Retinal Explant Culture
- 03:36Fixing and Cryo‐Sectioning
- 05:01Cyclic Guanosine Monophosphate (cGMP) Staining
- 06:13TUNEL Staining
- 07:58cGMP Staining Combined with TUNEL Staining
- 08:40Results: Macaque Monkey Retinal Explant Culture
- 09:20Conclusion
Retinal explants obtained from wild-type macaques were cultured in vitro. Retinal degeneration and the cGMP-PKG signaling pathway was induced using the PDE6 inhibitor zaprinast. cGMP accumulation in the explants at different zaprinast concentrations was verified using immunofluorescence.
