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Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Article DOI: 10.3791/64195-v • 08:19 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1, 1, 2, 1, 2
1Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis.

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CRISPR/Cas9 Mutagenesis Oriental Fruit Fly Bactrocera Dorsalis Functional Gene Research Pest Management Strategies Protocol Mutant Flies Target Genes Exons Introns Bioinformatic Analysis GRNA Synthesis Kit SgRNA Concentration UV-visible Spectrophotometer Rearing Condition Pupae Plastic Cage Relative Humidity Light/day Cycle Adults Eclose Sugar And Yeast Mixture Sexual Maturity Light Stand Fecundity Of Females Embryo Collection Oviposition Chamber
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