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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
JoVE 杂志
生物学
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JoVE 杂志 生物学
Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
DOI:

06:12 min

March 17, 2023

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Chapters

  • 00:05Introduction
  • 00:48Gold Substrate Preparation
  • 01:35Surface Plasmon Resonance Imaging
  • 03:04Atomic Force Microscopy
  • 03:52Results: Characterization of Extracellular Vesicles (EVs) on a Biochip
  • 05:28Conclusion

Summary

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This paper proposes a new generation of multiparametric analytical platforms with increased throughput for the characterization of extracellular vesicle subsets. The method is based on a combination of multiplexed biosensing methods with metrological and morphomechanical analyses by atomic force microscopy, coupled with Raman spectroscopy, to qualify vesicular targets trapped on a microarray biochip.

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