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Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

Article DOI: 10.3791/64274-v • 11:00 min • June 23rd, 2023
June 23rd, 2023 •
1,2, 1
1Children’s Mercy Research Institute, Children’s Mercy Kansas City, 2Department of Pediatrics, University of Missouri-Kansas City School of Medicine

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Many upregulated genes stimulate tumor cell migration and invasion, leading to poor prognosis. Determining which genes regulate tumor cell migration and invasion is critical. This protocol presents a method for investigating the effects of the increased expression of a gene on the migration and invasion of tumor cells in real time.

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Cancer Research Tumor Cell Migration Invasion Synthetic MRNA Transfection Gene Expression Patterns Tumor Cell Infiltration Metastasis Gene Knockdown Impedance-based Measurement MRNA Vaccines Therapeutic Purposes Gene Overexpression Synthetic MRNA Transfection Stimulation Method Paper Altered Gene Expression
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