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Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome
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Fast Colony Forming Unit Counting in 96-Well Plate Format Applied to the Drosophila Microbiome

Article DOI: 10.3791/64298-v • 12:55 min • January 13th, 2023
January 13th, 2023 •
1, 1,2
1Department of Embryology, Carnegie Institution for Science, 2Department of Biology, Johns Hopkins University

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This method quantifies microbial abundance using a 96-well plate format to plate colony forming units (CFUs) and is applied to the Drosophila microbiome in whole fly homogenate samples. CFUs are counted with an automated image analysis software provided here.

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Colony Forming Units CFU Counting Microbiology Agar Plates Fast Counting Method 96-well Plate Format Drosophila Microbiome Gut Microbiome Small Animal Models Fruit Flies Protocol DIY-fabricated Instrumentation Custom Software Plating Automation Manual Dexterity Automated Counting Software Threshold Comparison Ren Dodge Glass Beads PCR Plate
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