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Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
DOI:

06:26 min

January 06, 2023

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Chapters

  • 00:04Introduction
  • 00:37Sample Preparation for Fluorescent Size Exclusion Chromatography (FSEC)
  • 02:06Size Exclusion Chromatography (SEC)
  • 03:33Fluorescent Trace Collection and Analysis
  • 04:17Results: Fluorescent Size Exclusion Chromatography (FSEC) on Membrane Proteins for Downstream Functional and Structural Analysis Quality Assessment
  • 05:48Conclusion

Summary

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The present protocol describes a procedure to perform fluorescent size exclusion chromatography (FSEC) on membrane proteins to assess their quality for downstream functional and structural analysis. Representative FSEC results collected for several G-protein coupled receptors (GPCRs) under detergent-solubilized and detergent-free conditions are presented.

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