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Assessment of Ex Vivo Murine Biventricular Function in a Langendorff Model

Article DOI: 10.3791/64384-v • 07:54 min • December 23rd, 2022
December 23rd, 2022 •
1,2, 1, 1, 1, 1
1University of Michigan, Ann Arbor, 2University of Montreal

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Presented here is a protocol to reliably quantify the right and left ventricular function of donor hearts after cold preservation using an ex vivo perfusion system.

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Ex Vivo Murine Biventricular Function Langendorff Model Primary Graft Dysfunction Heart Transplantation Ischemic Time Cold Preservation Cardiac Function Evaluation Perfusion Histidine-tryptophan-ketoglutarate (HTK) Solution Kreb Buffer Silicone Balloon DP/dt Pressure-volume Relationships Heart Preservation Protocols Native Cardiac Cells Transgenic Mice Mechanisms Of PGD
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