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An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
DOI:

11:04 min

January 13, 2023

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Chapters

  • 00:02Introduction
  • 00:40Device Construction
  • 03:10Cell Lines: Cultivation, Seeding, and Treatment
  • 05:19Device Operation
  • 06:56Cell Viability Assays
  • 07:47Representative Results
  • 09:50Conclusion

Summary

自动翻译

Here, we describe a novel, simple, and low-cost device to successfully perform in vitro photodynamic therapy (PDT) assays using two-dimensional HeLa cell culture and verteporfin as a photosensitizer.

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