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An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

DOI:
10.3791/64391-v

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11:04 min

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January 13, 2023

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, , , , , , , , , , ,
1Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance,Federal University of Parana, 2Graduate Program in Pharmaceutical Sciences,Federal University of Parana, 3Federal Institute of Parana

Chapters

  • 00:02Introduction
  • 00:40Device Construction
  • 03:10Cell Lines: Cultivation, Seeding, and Treatment
  • 05:19Device Operation
  • 06:56Cell Viability Assays
  • 07:47Representative Results
  • 09:50Conclusion

Summary

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Here, we describe a novel, simple, and low-cost device to successfully perform in vitro photodynamic therapy (PDT) assays using two-dimensional HeLa cell culture and verteporfin as a photosensitizer.

Tags

Photodynamic TherapyPDTLight-emitting DiodeLEDVerteporfinCytotoxicity2D Cell CultureIn-house-built DevicePhotoActLow-costHeLa CellsDulbecco’s Modified Eagle MediumCell Culture

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