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Developmental Biology

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JoVE Journal Developmental Biology
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
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Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

Article DOI: 10.3791/64439-v • 08:24 min • November 11th, 2022
November 11th, 2022 •
1,2, 3, 1
1Animal Sciences Research Center, University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core, University of Missouri

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An optimized protocol is presented that enables the depletion of cytoplasmic microtubule organizing centers in mouse oocytes during metaphase I using a near-infrared femtosecond laser.

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Multi-Photon Laser Ablation Cytoplasmic Microtubule Organizing Centers Mouse Oocytes Oocyte Meiosis Nuclear Envelope Breakdown Spindle Positioning Kinetochore-microtubule Attachments Aneuploidy Centrosome-mediated Astral MTs Cell Cortex Acentriolar MT Organizing Centers Metaphase Cytoplasmic MTOCs Spindle Poles McMTOCs Multi-photon Laser Ablation Method
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