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Chemistry
使用 卤虫 盐L的致死性生物测定。
使用 卤虫 盐L的致死性生物测定。
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Lethality Bioassay Using Artemia salina L.

使用 卤虫 盐L的致死性生物测定。

Full Text
12,616 Views
09:09 min
October 11, 2022

DOI: 10.3791/64472-v

Márcia Santos Filipe*1,2, Vera M. S. Isca*1,3, Epole Ntungwe N.1,2, Salvatore Princiotto1, Ana Maria Díaz-Lanza2, Patrícia Rijo1,3

1CBIOS - Lusófona University's Center for Research in Biosciences & Health Technologies,Universidade Lusófona de Humanidades e Tecnologias, 2Facultad de Farmacia, Departamento de Ciencias Biomédicas (Área de Farmacologia; Nuevos agentes antitumorales, Acción tóxica sobre células leucémicas),Universidad de Alcalá de Henares, 3Instituto de Investigação do Medicamento (iMed.ULisboa),Faculdade de Farmácia da Universidade de Lisboa

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Please note that some of the translations on this page are AI generated. Click here for the English version.

这项工作旨在评估和审查 卤虫 盐碱致死性生物测定程序,也称为盐水虾致死性测定。这种简单而廉价的方法提供了有关样品(即天然产物)的一般毒性(被视为初步毒性评估)的信息。

该协议是对不同类型的样品(例如来自不同天然或合成来源的提取物,馏分和化合物)进行初步一般毒性评估的非常有用的工具。我们的实验方案的主要优点是它非常简单且便宜,可以同时用于许多样品。此外,手工制作的设备提高了现场的可靠性。

在这个技术阶段,我们进行筛选,根据其初步的一般毒性特征选择样品,这些样品将接受进一步的特定测试,例如膨胀细胞系甚至体内测试。该方法主要用于医药和其他产品化学领域,以筛选生物样品。我们的协议也可用于其他类型的样品,如纳米系统。

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