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High-Content Screening Assay for the Identification of Antibody-Dependent Cellular Cytotoxicity Modifying Compounds

Article DOI: 10.3791/64485-v • 13:59 min • August 18th, 2023
August 18th, 2023 •
*1,2, *1,3, *1,3, 1, 1,4, 1,4
1Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, 2Doctoral School of Molecular Medicine, University of Debrecen, 3National Academy of Scientist Education, University of Debrecen, 4ELKH-DE Cell Biology and Signaling Research Group
* These authors contributed equally

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This protocol presents an automated, image-based high-throughput technique to identify compounds modulating natural killer cell-mediated breast cancer cell killing in the presence of a therapeutic anti-HER-2 antibody.

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High-Content Screening Assay Antibody-Dependent Cellular Cytotoxicity ADCC NK Cells Granulocytes Macrophages Effector Cells Trastuzumab HER2 FcyR Receptors Cytotoxic Granules Apoptosis Cancer Cells JIMT-1 Breast Carcinoma Cells NK-92 Cell Line Green Fluorescent Protein 96-well High-content Screening Plate JIMT Medium CO2 Incubator Trypsinize Cells Sterile PBS
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