Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Ashton E. Enrriques; Sean Howard; Raju Timsina; Nawal K. Khadka; Amber N. Hoover; Allison E. Ray; Ling Ding; Chioma Onwumelu; Stephan Nordeng; Laxman Mainali; Gunes Uzer; Paul  H. Davis

doi:10.3791/64497

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Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

原子力显微镜悬臂式纳米压痕:空气和流体中纳米级机械性能测量

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08:58 min

December 2, 2022

DOI: 10.3791/64497-v

Ashton E. Enrriques¹, Sean Howard², Raju Timsina³, Nawal K. Khadka³, Amber N. Hoover⁴, Allison E. Ray⁵, Ling Ding⁴, Chioma Onwumelu⁶, Stephan Nordeng⁶, Laxman Mainali^3,7, Gunes Uzer², Paul H. Davis^1,8

¹Micron School of Materials Science & Engineering,Boise State University, ²Department of Mechanical & Biomedical Engineering,Boise State University, ³Department of Physics,Boise State University, ⁴Energy and Environmental Science and Technology,Idaho National Laboratory, ⁵Science and Technology,Idaho National Laboratory, ⁶Harold Hamm School of Geology & Geological Engineering,University of North Dakota, ⁷Biomolecular Sciences Graduate Program,Boise State University, ⁸Center for Advanced Energy Studies

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量化原子力显微镜（AFM）探针尖端施加到样品表面的接触面积和力，可以确定纳米级机械性能。讨论了在软硬样品的空气或流体中实施基于AFM悬臂的纳米压痕的最佳实践，以测量弹性模量或其他纳米力学性能。

原子力显微镜或基于AFM悬臂的纳米压痕可用于确定材料在空气和流体中从千帕斯卡到千兆帕斯卡的模量的纳米级机械性能。基于AFM悬臂的纳米压痕能够在各种材料和相关环境中以纳米级精度和分辨率进行共定位形貌成像和原位定量机械性能测量。基于AFM悬臂的纳米压痕可用于区分表现出不同机械性能的健康与疾病结构，组织或细胞。

准确确定尖端样品接触面积和在悬臂式纳米压痕过程中施加的力需要仔细校准AFM探头，这对于定量纳米级机械性能测量具有挑战性，但至关重要。首先，根据介质、预期模量、样品形貌和相关特征尺寸选择合适的原子力显微镜或AFM探针，对目标样品进行纳米压痕。将探头装载到探头支架上，并将探头支架连接到AFM扫描头。

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