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Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli
JoVE 杂志
遗传学
This content is Free Access.
JoVE 杂志 遗传学
Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

DOI:
10.3791/64523-v

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06:56 min

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March 24, 2023

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, , , , , ,
1School of Biological Sciences,University of California Irvine, 2Department of Microbiology & Environmental Toxicology,University of California Santa Cruz

Chapters

  • 00:05Introduction
  • 01:15Conjugation
  • 03:16Polymerase Chain Reaction (PCR) and Agarose Gel Electrophoresis
  • 05:10Results: Conjugative Plasmid Detection and Transfer
  • 06:04Conclusion

Summary

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Conjugation mediates horizontal gene transfer by mobilizing plasmid DNA across two different cells, facilitating the spread of beneficial genes. This work describes a widely-used method for the efficient detection of conjugative plasmid transfer, based on the use of differential markers in the conjugative plasmid, donor, and recipient to detect transconjugation.

Tags

Horizontal Gene TransferConjugative PlasmidsE. ColiAntibiotic ResistanceEpidemiological SurveillanceMating AssayDonorRecipientSelective Media

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