Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

Hannah E. Korah; Kevin Cheng; Stephanie M. Washington; Matthew E. Flowers; Harrison J. Stratton; Amol Patwardhan; Mohab M. Ibrahim; Laurent F. Martin

doi:10.3791/64555

JoVE Journal
Medicine

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JoVE Journal Medicine

Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

部分坐骨神经结扎术:慢性神经性疼痛的小鼠模型，用于研究新疗法的抗伤害感受作用

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08:16 min

October 6, 2022

DOI: 10.3791/64555-v

Hannah E. Korah¹, Kevin Cheng¹, Stephanie M. Washington², Matthew E. Flowers¹, Harrison J. Stratton¹, Amol Patwardhan⁵, Mohab M. Ibrahim*^1,2,3,4, Laurent F. Martin*^1,2

¹Department of Pharmacology, College of Medicine,The University of Arizona, Tucson, ²Department of Anesthesiology, College of Medicine,The University of Arizona, Tucson, ³Neuroscience Graduate Interdisciplinary Program, College of Medicine,The University of Arizona, Tucson, ⁴Comprehensive Pain and Addiction Center,The University of Arizona, Tucson, ⁵Department of Anesthesiology and Pain Management,University of Texas Southwestern Medical Center

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坐骨神经部分结扎可诱发长期慢性神经性疼痛，其特征是对热和机械刺激的夸张反应。这种神经性疼痛的小鼠模型通常用于研究疼痛管理的创新疗法。本文详细介绍了提高标准化和可重复性的外科手术。

由于现在正在研究大量用于治疗慢性疼痛的创新疗法，我们的协议为诱导神经性疼痛所需的手术标准化提供了关键概念。部分坐骨神经结扎术（PSNL）是研究神经性疼痛的有利模型，因为它再现了在患有神经性疼痛的人类中观察到的许多症状。由于PSNL再现了在患有神经性疼痛的人类中观察到的许多症状，因此该协议为研究和开发新的疼痛管理疗法提供了关键概念。

演示该程序的将是Kevin Cheng，我实验室的医学博士候选人。首先，在测试之前，将小鼠放在与测试室同一房间的金属丝网上的透明有机玻璃盒中，使小鼠习惯于Von Frey测试设备一小时。将一系列校准的细单丝垂直于悬挂在吊丝网笼中的动物的PSNL同侧后爪的播种机表面。

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