Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

Ricardo Gon&#231;alves; Gabriela Kaliff Te&#243;filo Murta; Izabela Aparecida de Souza; David M. Mosser

doi:10.3791/64566

JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

从小鼠中分离和培养骨髓来源的巨噬细胞

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07:12 min

June 23, 2023

DOI: 10.3791/64566-v

Ricardo Gonçalves¹, Gabriela Kaliff Teófilo Murta¹, Izabela Aparecida de Souza¹, David M. Mosser²

¹Department of General Pathology, Institute of Biological Sciences,Federal University of Minas Gerais, ²Department of Cell Biology and Molecular Genetics,University of Maryland

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本方案描述了从小鼠中分离和培养骨髓来源的巨噬细胞。

该方案旨在获得大量源自骨髓且从未暴露于组织因子或细胞因子的未刺激巨噬细胞。该技术的主要优点是从单个动物获得的细胞数量。通过正确的程序，可以获得大约 2 乘以 10 的 7 个巨噬细胞的幂。

这种方法可以帮助来自不同领域的研究人员研究巨噬细胞，如细胞生物学、病理学、免疫学和寄生虫学，因为细胞总是容易受到污染，所以对所有无菌步骤都要小心并使用层流生物安全柜来维持细胞活力。演示该程序的是伊莎贝拉·阿帕雷西达·德·索萨（Izabela Aparecida de Souza）。我们实验室的硕士研究生。

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