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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
DOI:

10:00 min

November 30, 2022

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Chapters

  • 00:05Introduction
  • 00:53Transposase mRNA Preparation
  • 03:07Multisite Gateway Cloning to Create Entry Vectors for Transgenesis
  • 05:44Injection of the Transgene into the Embryos
  • 07:00Screening Embryos for Transgenic Insertion
  • 07:35Results: Transgenic Constructs of Zebrafish Transgenesis and Their Use in Ethanol Studies
  • 09:34Conclusion

Summary

自动翻译

This work describes a protocol for the modular Tol2 transgenesis system, a gateway-based cloning method to create and inject transgenic constructs into zebrafish embryos.

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