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Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Article DOI: 10.3791/64688-v • 05:34 min • February 10th, 2023
February 10th, 2023 •
*1, *1,3, 2, 4, 1,3, 1,3, 1,3
1State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, 2Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, College of Fishery, Guangdong Ocean University, 3Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, 4Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University
* These authors contributed equally

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Here, we demonstrate the preparation of a high-quality single-cell suspension of tilapia intestine for single-cell sequencing.

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Single-cell Suspension Preparation Nile Tilapia Intestine Single-cell Sequencing Aquaculture Fish Species Laboratory Conditions Enzymatic Cell Dissociation Reagent Collagenase Dispase PBS FBS Centrifuge Tissue Dissection Intestines Midsection Fat And Mesentery Excision Clear Mucosa Removal Sterile Ice Cold PBS Syringe Small Pieces Transfer 1.5 Milliliter Tube Centrifugation
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