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JoVE Journal Biochemistry
Concanavalin A-basierter Sedimentationsassay zur Messung der Substratbindung von Glucanphosphatasen
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Concanavalin A-basierter Sedimentationsassay zur Messung der Substratbindung von Glucanphosphatasen

Article DOI: 10.3791/64700-v • 09:07 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1, 1, 1, 1
1Department of Chemistry, Skidmore College
* These authors contributed equally

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Diese Methode beschreibt einen lektinbasierten in vitro Sedimentationsassay zur Quantifizierung der Bindungsaffinität von Glucanphosphatase und Amylopektin. Dieser Co-Sedimentations-Assay ist zuverlässig für die Messung der Glucan-Phosphatase-Substratbindung und kann auf verschiedene solubilisierte Glucan-Substrate angewendet werden.

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