利用改良酶联免疫吸附测定法检测和定量人血浆中降钙素基因相关肽（CGRP） 关键词：降钙素基因相关肽，CGRP，酶联免疫吸附测定，ELISA，人血浆，肽检测，定量分析
4K 次观看
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被引用 4 次
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07:14 分钟
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2023年6月16日
DOI :10.3791/64775-v
2023年6月16日
•4K 次观看
Pavan S. Krishnan1,2 , Fernando T. Zamuner1,3 , Carolyn M. Jenks1 , Johnny Y. Xie4 , Lisa Zhang5 , Mohammed Lehar1 , Neal S. Fedarko6 , Mariana Brait1,3 , John P. Carey1
1Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine
关于人血浆中降钙素基因相关肽（CGRP）浓度的已发表数据存在不一致。这种不一致可能是由于缺乏标准化且经过验证的定量该神经肽的方法学所致。本文中，我们描述了一种经过验证的酶联免疫吸附测定（ELISA）方案，用于纯化和定量人血浆中的CGRP。
Chapters in this video
0:04
Introduction
1:03
Collection, Preparation, and Extraction of Plasma Sample
3:28
ELISA Plate Preparation (Blocking)
4:16
Enzyme-Linked Immunosorbent Assay (ELISA)
6:06
Results: Effect of Blocking on CGRP Quantification
6:29
Conclusion
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