A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

Irisdoris Rodrigues de Souza; Andrezza Di Pietro Micali Canavez; Desiree Cigaran Schuck; Viviana Stephanie Costa Gagosian; Isisdoris Rodrigues de Souza; Nat&#225;lia de Albuquerque Vita; Edvaldo da Silva Trindade; Marta Margarete Cestari; Marcio Lorencini; Daniela Morais Leme

doi:10.3791/64859

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A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines

胚胎和肝脏斑马鱼细胞系球状体的3D培养方法

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January 20, 2023

DOI: 10.3791/64859-v

Irisdoris Rodrigues de Souza¹, Andrezza Di Pietro Micali Canavez², Desiree Cigaran Schuck², Viviana Stephanie Costa Gagosian¹, Isisdoris Rodrigues de Souza¹, Natália de Albuquerque Vita², Edvaldo da Silva Trindade³, Marta Margarete Cestari⁴, Marcio Lorencini², Daniela Morais Leme⁴

¹Graduate Program in Genetics, Department of Genetics,Federal University of Paraná (UFPR), ²Safety of Product Department,Grupo Boticário, ³Department of Cell Biology,Federal University of Paraná (UFPR), ⁴Department of Genetics,Federal University of Paraná (UFPR)

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在这里，我们提出了一种有效，简单和快速的3D培养方案，用于形成两种斑马鱼（Danio rerio）细胞系的球状体:ZEM2S（胚胎）和ZFL（正常肝细胞）。

该协议可以轻松生成斑马鱼球状体，这是一种3D体外模型，在鱼类毒性测试中具有不同的潜在应用。这是一种简单快速的方法，可以生成大小和形状非常相似的斑马鱼细胞系的单个球状体。如果您正确执行所有描述的步骤，则应该不会遇到任何问题。

斑马鱼球状体提供的机理，探索化学物质对鱼类的影响，有助于提高化妆品等产品的环境安全评价。展示该程序的将是Irisdoris Rodrigues de Souza，我实验室的遗传学博士。首先，取一瓶T75烧瓶的斑马鱼细胞，汇合度约为80%。

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撤稿第 191 期