The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation

Camille Hossay; Luciana Cacciottola; Sarah Storder; Olivier Van Kerk; Marie-Madeleine Dolmans

doi:10.3791/64867

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Biology

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The Chick Chorioallantoic Membrane (CAM) Model as a Tool to Study Ovarian Tissue Transplantation

鸡绒毛膜尿囊膜（CAM）模型作为研究卵巢组织移植的工具

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05:38 min

June 23, 2023

DOI: 10.3791/64867-v

Camille Hossay¹, Luciana Cacciottola¹, Sarah Storder¹, Olivier Van Kerk¹, Marie-Madeleine Dolmans^1,2

¹Gynecology Research Unit, Institut de Recherche Expérimentale et Clinique,Université Catholique de Louvain, ²Gynecology Department,Cliniques Universitaires Saint-Luc

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Overview

This study presents a protocol for establishing a chick chorioallantoic membrane (CAM) xenografting model to investigate human ovarian tissue transplantation. By utilizing the CAM system, the research demonstrates effective graft revascularization and tissue viability over a six-day period, revealing insights into early vascularization and follicle survival.

Key Study Components

Research Area

Ovarian tissue transplantation
Vascularization mechanisms
Follicle survival

Background

The advantages of CAM over in vitro models
Development of skills in egg manipulation
Ethical considerations addressed

Methods Used

Chick chorioallantoic membrane (CAM) model
Embryonated chick eggs for grafting
Digital imaging and histological evaluation

Main Results

Successful grafts maintained 83% survival rate after six days
Good vascularization of grafts observed, with 80% penetration into the egg
Follicle densities remained relatively stable during grafting

Conclusions

This model effectively allows for the study of early events in ovarian tissue transplantation
It holds significant relevance for understanding factors that affect follicle activation and vascularization

Frequently Asked Questions

What is the significance of using the CAM model in this research?

The CAM model allows observation of early vascularization and follicle loss without ethical concerns and without graft rejection, providing a dynamic in vivo environment.

How does the CAM model enhance our understanding of ovarian tissue transplantation?

It enables the exploration of factors influencing vascularization and follicle survival in a controlled environment, crucial for improving transplantation techniques.

What are the main challenges faced during the grafting procedure?

The most critical challenge is creating a small hole in the egg to aspirate albumin without damaging the CAM or the embryo.

What were the rates of follicle survival observed in this study?

Follicle survival rates were maintained at 95% on day one and 83% on day six post-grafting.

How does this research contribute to future ovarian tissue research?

The findings provide insights into the mechanisms of early grafting events, paving the way for testing pro-angiogenic factors and other influences on follicle health.

Is this method cost-effective for labs with limited resources?

Yes, the CAM model is relatively inexpensive and does not require complex setups, making it accessible for various research facilities.

What future experiments can be derived from this study's findings?

Future experiments could explore the impact of specific growth factors and hormones on follicle activation and vascularization in ovarian tissue.

在这里，我们描述了为人类卵巢组织开发鸡绒毛膜尿囊膜（CAM）异种移植模型的方案，并展示了该技术的有效性、移植物血运重建时间范围以及 6 天移植期间的组织活力。

与体外模型相比，使用 CAM 观察卵巢组织移植后的早期变化具有显着优势，因为它可以研究影响早期血管形成和卵泡丢失过程的机制。这种技术价格低廉，并且可以在前 17 天内由于天然胚胎免疫缺陷而没有任何移植物排斥的风险。此外，这种方法在欧洲法律方面不会引起任何道德或法律问题。

在实际实验之前，可以通过对未胚胎的卵子进行训练来获得开发卵子操作所需的手动技能的最佳策略。首先，使用记号笔标记从 Louvain 选定的白色长角雏鸡那里收到的零蛋日。在设置为37摄氏度的孵化器中孵化鸡蛋，尖头朝下。

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生物学第 196 期卵泡活化卵泡丢失冷冻保存移植啮齿动物模型替代方案成本效益省时伦理考虑异种移植人类卵巢组织免疫缺陷血管生成移植后卵泡丢失过程 CAM 异种移植模型血运重建时间范围组织活力