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Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings
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神经科学
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JoVE 杂志 神经科学
Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings

Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings

DOI:
10.3791/64989-v

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09:39 min

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March 17, 2023

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, , ,
1Department of Anatomy, Instituto de Ciencias Biomedicas,Universidade de Sao Paulo

Chapters

  • 00:05Introduction
  • 00:48Brain Dissection and Slicing
  • 02:49Cell Sealing for Patch‐Clamp Recording
  • 07:29Results: Whole‐Cell Patch‐Clamp Recordings on the Brain Slices
  • 08:39Conclusion

Summary

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Here, we present a protocol to perform a whole-cell patch-clamp on brain slices containing kisspeptin neurons, the primary modulator of gonadotrophin-releasing hormone (GnRH) cells. By adding knowledge about kisspeptin neuron activity, this electrophysiological tool has served as the basis for significant advancements in the neuroendocrinology field over the last 20 years.

Tags

Whole-cell Patch-clamp RecordingsElectrophysiological PropertiesCell MembranesIonic CurrentsCellular ResponseBrain DissectionHypothalamusOptic NerveVibratomeACSF SolutionMicroscopeAmplifierDigitizerMicromanipulatorPerfusion Pump

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