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Monitoring Stub1-Mediated Pexophagy
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生物学
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JoVE 杂志 生物学
Monitoring Stub1-Mediated Pexophagy

Monitoring Stub1-Mediated Pexophagy

DOI:
10.3791/65010-v

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08:26 min

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May 12, 2023

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,
1Institute of Biological Chemistry,Academia Sinica, 2Institute of Biochemical Sciences, College of Life Sciences,National Taiwan University

Chapters

  • 00:05Introduction
  • 00:51Preparation of Cells Expressing DiKillerRed or Self‐Labeling Proteins (SLPs) in the Peroxisome Lumen
  • 02:52Staining Peroxisomes with Dye‐Labeled SLP Ligands
  • 03:39ROS‐Stressing of Peroxisomes on a Laser‐Scanning Confocal Microscope
  • 06:25Results: Light‐Activated ROS Production and Stub1‐Mediated Pexophagy
  • 07:50Conclusion

Summary

自动翻译

自动翻译

This protocol provides instructions for triggering and monitoring Stub1-mediated pexophagy in live cells.

Tags

PeroxisomeROSOrganelle InjuryCell CultureTransfectionLight-activated ROSHaloTagConfocal Microscopy

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