Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Adipocyte-Specific ATAC-Seq with Adipose Tissues…
JoVE Journal
Genetics

需要订阅 JoVE 才能查看此 内容.

JoVE Journal Genetics
Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)
Journal (Genetics)
Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)
CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation
Journal (Genetics)
CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus (AAV) and 2-Cell Embryo Electroporation
 

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Article DOI: 10.3791/65033-v • 11:11 min • March 17th, 2023
March 17th, 2023 •
1, 1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine

章节

总结概括
Automatic Translation

We present a protocol for assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) specifically on adipocytes using nucleus sorting with adipose tissues isolated from transgenic reporter mice with nuclear fluorescence labeling.

Tags

Adipocyte-Specific ATAC-Seq Adipose Tissues Fluorescence-Activated Nucleus Sorting Gene Expression Regulatory Mechanisms Lipid Contents Mitochondrial Contamination Cellular Heterogeneity Adipocyte-specific ATAC-sequencing High-quality Data Mitochondrial DNA Contamination Nucleus Isolation Glass Dounce Homogenizers NPB Mix Frozen Adipose Tissue Scissors Loose Pestle Tight Pestle 100 Micrometer Strainer 50 Milliliter Tube Centrifugation Supernatant PBS-N Solution 40 Micrometer Strainer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter