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Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis
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Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis

Article DOI: 10.3791/65054-v • 09:24 min • March 17th, 2023
March 17th, 2023 •
1, 1, 1, 1, 1, 2, 1
1State Key Laboratory of Southwestern Chinese Medicine Resources, Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, 2School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine

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Here, we describe a general protocol and design that could be applied to identify trace amounts and minor constituents in the complex natural product formulations (matrixes) in Tibetan medicine.

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Standardized Identification Compound Structure Tibetan Medicine Ion Trap Mass Spectrometry Multiple-stage Fragmentation Analysis Qualitative Study Natural Medicine Accuracy Database Metabolic Intermediates Bladder Urine Abelmoschus Manihot's Seeds Sample Preparation Extraction Ultrasound Bath Sonicator Centrifuge Injection Syringe Microporous Membrane Filter Vacuum Pump Argon Cylinder Nitrogen Valve Mass Spectrometry (MS) Control Software Heated ESI Source
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