A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

Haifeng Shao; Jialin Deng; Yanqing Liu; Niuniu Yang

doi:10.3791/65120

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

用于过敏性接触性皮炎评估的小鼠耳模型

Full Text

4,993 Views

08:02 min

March 24, 2023

DOI: 10.3791/65120-v

Haifeng Shao^1,2,3,4, Jialin Deng^2,3,4, Yanqing Liu^1,2,3,4, Niuniu Yang^1,2,3,4

¹Department of Traditional Chinese Medicine, The Affiliated Hospital of Yangzhou University,Yangzhou University, ²Department of Traditional Chinese and Western Medicine, Medical College,Yangzhou University, ³The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine,Yangzhou University, ⁴Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Yangzhou University

Please note that some of the translations on this page are AI generated. Click here for the English version.

在这里，我们描述了通过1-氟-2，4-二硝基苯（DNFB）诱导小鼠耳朵过敏性接触性皮炎的方法以及如何评估过敏性接触性皮炎的严重程度。

该模型不仅可用于探索ACD的发病机制，而且适用于新治疗方法的临床前进化，具有重要意义。这是一种稳定高效的动物模型，具有实验周期短、操作方便、症状明显、成功率高等诸多优点。我们的实验方案非常详细且易于操作。

确保正确遵循协议的每个步骤。在开始建模之前，让小鼠适应环境一周，自由获得食物和水。在建模当天，使用紫外线灯和75%酒精，清洁和消毒环境和台面。

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

本月在JoVE上第193期过敏性接触性皮炎炎症 DNFB